Standard Test Method for
Retention Characteristics of 0.2-µm Membrane Filters Used
in Routine Filtration Procedures for the Evaluation of
Microbiological Water Quality1
This standard is issued under the fixed designation D 3862; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of the last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1.1 This test method covers a procedure to test membrane
filters for their ability to retain bacteria whose diameter is equal
to or slightly larger than the 0.2-µm pore size of the membrane
1.2 The procedures described are for the use of the user
laboratories as differentiated from manufacturers’ laboratories.
1.3 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents
2.1 ASTM Standards:
D 1129 Terminology Relating to Water2
D 1193 Specification for Reagent Water2
3.1.1 For definitions of terms used in this test method, refer
to Terminology D 1129.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 Gram stain—a routine bacterial stain that divides
bacteria into two categories, depending on whether they can be
decolorized with acetone, alcohol, or aniline oil after staining
with one of the rosaniline dyes such as crystal violet, methyl
violet, or gentian violet and treating with iodine. Those that
resist decolorization remain blue or violet and are designated
Gram-positive; those that are decolorized and take up the red
counterstains, such as neutral red, safranin, or dilute carbon
fuchsin is termed Gram-negative.
3.2.2 vacuum—for the procedure used—the source of suction
that can produce a reading of 500 to 600 mm Hg on a vacuum
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