Standard Test Method for
Adenosine Triphosphate (ATP) Content of Microorganisms
This standard is issued under the fixed designation D 4012; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of the last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1.1 This test method covers the measurement of adenosine
triphosphate (ATP) in microorganisms in concentrations normally found in laboratory cultures, waters, wastewaters, and in
plankton and periphyton samples from waters.
1.2 Knowledge of the concentration of ATP can be related to
viable biomass or metabolic activity, or by utilizing an average
concentration (or amount) of ATP per cell, an estimated count
of microorganisms can be obtained in the case of unit species
1.3 This test method offers a high degree of sensitivity,
rapidity, accuracy, and reproducibility. However, extreme care
must be taken at each step in the analysis to ensure meaningful
and reliable results.
1.4 The analyst should be aware that the precision statement
pertains only to determinations in reagent water and not
necessarily in the matrix being tested.
1.5 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents
2.1 ASTM Standards:
D 1129 Terminology Relating to Water2
D 1193 Specification for Reagent Water2
3.1 Definitions—For definitions of terms used in this test
method, refer to Terminology D 1129.
4. Summary of Test Method
4.1 The biomass in the sample can be determined by direct
ATP extraction when cell counts are greater than 20 000
Click below to download Astm D 4012 – 81(Reapproved 2002) pdf free
Click here to download Astm D 4009 – 92 (Reapproved 1997) Pdf Free Download