Standard Practice for
Sampling Phytoplankton with Water-Sampling Bottles1
This standard is issued under the fixed designation D 4136; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of the last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This practice covers the procedures for obtaining quantitative samples of a phytoplankton community by use of
water-sampling bottles.
1.2 This standard does not purport to address all of the
safety problems, if any, associated with its use. It is the
responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents
2.1 ASTM Standards: 2
D 4137 Practice for Preserving Phytoplankton Samples
3. Summary of Practice
3.1 A sampler is lowered to a specified depth and closed by
a messenger. The sampler is retrieved and the sample is
removed. The phytoplankton is preserved as dictated by the
objectives of the study.
4. Significance and Use
4.1 The advantages of water-sampling bottles are as follows:
4.1.1 Samples of known volume can be obtained from a
precise depth.
4.1.2 A quantitative sample is obtained. Nanno- and ultraplankton are not lost from the sampling device.
4.1.3 A sampler of almost any desired volume or construction material can be obtained.
4.1.4 The sampler is lightweight and can be used without
auxiliary equipment.
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