Standard Test Method for
Evaluating Inhibitory Effects of Ink Grids on Membrane
This standard is issued under the fixed designation D 4200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of the last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1.1 This test method describes a procedure whereby the user
of ink-gridded membrane filters in water quality studies can
ascertain whether or not the grid lines are toxic and inhibitory
to bacterial growth when the membrane and its entrapped
bacteria are incubated on a suitable media.
1.2 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents
2.1 ASTM Standards: 2
D 1129 Terminology Relating to Water
D 1193 Specification for Reagent Water
3.1 Definitions—For definitions of terms used in this test
method, refer to Terminology D 1129.
4. Summary of Test Method
4.1 A heavy bacterial suspension is filtered through a
gridded membrane filter. The bacterial concentration employed
is sufficient to cover much of the membrane with bacterial
4.2 After filtration the membrane is incubated on a suitable
medium and the distribution of the colonies and the shape of the
colonies are noted in the area around each grid line.
5. Significance and Use
5.1 This test method may be applied to determine the
suitability of grid-marked membrane filters for use in bacte
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